Ypt/Rab GTPases in Intersections of Cellular Trafficking and Autophagy Pathways. From yeast modeling to human disease Trafficking pathways, in which proteins and membranes are transported between cellular compartments, are vital for the proper functioning of all eukaryotic cells. In the secretory pathway, cargo is delivered from the endoplasmic reticulum to the plasma membrane (PM). Two major pathways lead to the cellular degradative compartment termed lysosome: Endocytosis delivers cargo from the PM and the cellular environment, whereas constitutive and stress-induced autophagy shuttles excess or damaged cellular components packed in autophagosomes for recycling. The conserved Ypt/Rab GTPases together with their GEF activators have emerged as key regulators of the multiple intracellular trafficking pathways. They are implicated in human disorders ranging from cancer to neurodegenerative diseases. Our long-term goal is to elucidate how the yeast Ypts and their human Rab homologs coordinate individual transport steps in the same pathway and in different pathways.
– Segev Project1
– Segev Project2