The Chronis lab is committed to determining the mechanisms that regulate Stem Cell differentiation and maintenance in both normal development and disease. We have been among the first to delineate the mechanisms by which nuclear transcription factors can ‘reverse development’ of somatic tissues to a Stem Cell like state in the form of iPSCs and are currently applying our iPSC technology for disease modeling and personalized therapies. We further develop and apply next generation sequencing, genome engineering, single cell epigenomics and computational approaches to translate our discoveries into new therapeutic options for patients.
Current rotation projects:
- Reprogramming the next generation of immunotherapies
In this rotation project we aim to reprogram somatic tissues to hematopoietic stem and progenitor cells using nuclear transcription factors and to uncover the mechanisms that govern such transitions. The produced cells will be used to model hematological malignancies and as a platform to test stage-specific genetic and pharmacological interventions.
- Developing New Molecular Methods to uncover the genetic Basis of Human Disease
This rotation project involves the development of novel methodologies to profile chromatin structure and function in normal and diseased states at the single cell level. The rotating student will employ next-generation DNA sequencing (ChIP-seq, ATAC-seq, RNA-seq) to benchmark our new method and explore mechanisms of gene regulation in stem cell maintenance and differentiation.