Rotation Projects

Project 1: Determine how hepatocyte-specific PPARgamma regulates lipid and amino acid metabolism in non-alcoholic steatohepatitis.
References:
Kineman et al. Endocrinology.2016.https://www.ncbi.nlm.nih.gov/pubmed/26950202

Journal of Endocrinology. 2017 https://www.ncbi.nlm.nih.gov/pubmed/27799461

Cordoba-Chacon J. PPAR Res. 202 https://www.ncbi.nlm.nih.gov/pubmed/32411189

Cellular and Molecular Life Sciences 2021. https://www.ncbi.nlm.nih.gov/pubmed/33444819

Project 2: Determine how conjugated linoleic acid regulates hepatic glucose production.
References:
Cordoba-Chacon J, et al. Journal of Nutritional Biochemistry 2019.https://www.ncbi.nlm.nih.gov/pubmed/30856468

Methods: Mouse models. Alteration of cell-specific expression in adult mice [before and after the development of the disease]. Assessment of glucose and lipid metabolism, and metabolic activity in vivo. Molecular biology techniques: qRT-PCR: absolute copy number. Western-blot. Mitochondrial activity. Gas chromatography/mass spectrometry. Cell culture and assessment of glucose and lipid metabolism in vitro. Hepatic cell lines and mouse primary hepatocytes. Histological characterization of liver pathology.

– Cordoba Lab GEMS

References:
– Paper with hepatocyte-specific PPARgamma knockout mouse model: https://www.ncbi.nlm.nih.gov/pubmed/27799461
– Abstract submitted to the 2019 Midwest Clinical and Translational Meeting (Page: 893-894): https://jim.bmj.com/content/jim/67/5/862.full.pdf
– Paper with the t10c12-CLA-induced lipodystrophy: https://www.ncbi.nlm.nih.gov/pubmed/30856468
Link to Cordoba Lab Website:https://cordoba.red.uic.edu/

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