Protocols
Handling and Assessment of Human Primary Prostate Organoid Culture
Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding,…
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
The goal of this protocol is to use laser-capture micro-dissection as an effective method to isolate pure populations of cell types…
Selected Publications
- The double disparity: Vitamin D deficiency and lethal prostate cancer in black men.
Duraki A, Krieger KD, Nonn L.J Steroid Biochem Mol Biol. 2025
- Next-generation sequencing identifies the presence of protein phosphatase 1D, PPM1D, as a potential biomarker of resistance to PARP inhibition in metastatic castration-resistant prostate cancer.
Vellky JE, Kirkpatrick BJ, Gutgesell LC, Kregel S, Tawagi K, Nuccio L, Vander Griend DJ, Nonn L, Reizine N.Am J Clin Exp Urol. 2025
- ERBB3 overexpression is enriched in diverse patient populations with castration-sensitive prostate cancer and is associated with a unique AR activity signature
Vellky JE, Kirkpatrick BJ, Gutgesell LC, Morales M, Brown R, Wu Y, Maienschein-Cline M, Notardonato LD, Weinfeld MS, Nguyen RS, Brister E, Sverdlov M, Liu LC, Xu Z, Kregel S, Nonn L, Vander Griend DJ, Reizine N.
Clin Cancer Res, 2024 - Prostate-derived circulating microRNAs add prognostic value to prostate cancer risk calculators.
Zenner ML, Kirkpatrick B, Leonardo TR, Schlicht MJ, Saldana AC, Loitz C, Valyi-Nagy K, Maienschein-Cline M, Gann PH, Abern M, Nonn L.
JEX Biology, in press, November 2023. https://doi.org/10.1002/jex2.122 - African American Prostate Cancer Displays Quantitatively Distinct Vitamin D Receptor Cistrome-transcriptome Relationships Regulated by BAZ1A.
Siddappa M, Hussain S, Wani SA, White J, Tang H, Gray JS, Jafari H, Wu HC, Long MD, Elhussin I, Karanam B, Wang H, Morgan R, Hardiman G, Adelani IB, Rotimi SO, Murphy AR, Nonn L, Davis MB, Kittles RA, Hughes Halbert C, Sucheston-Campbell LE, Yates C, Campbell MJ.
Cancer Res Commun. 2023 Apr 18;3(4):621-639. doi: 10.1158/2767-9764.CRC-22-0389. eCollection 2023 Apr.
PMID: 37082578 - Regulation of Prostate Androgens by Megalin and 25-hydroxyvitamin D Status: Mechanism for High Prostate Androgens in African American Men.
Garcia J, Krieger KD, Loitz C, Perez LM, Richards ZA, Helou Y, Kregel S, Celada S, Mesaros CA, Bosland M, Gann PH, Willnow TE, Vander Griend D, Kittles R, Prins GS, Penning T, Nonn L.
Cancer Res Commun. 2023 Mar 3;3(3):371-382. doi: 10.1158/2767-9764.CRC-22-0362. eCollection 2023 Mar.
PMID: 36875158 - Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures Perez L and Nonn L.
Front Oncol. 2022 Mar 31;12:864723. doi: 10.3389/fonc.2022.864723. eCollection 2022.
PMID: 35433436 - Single-cell RNA-Seq identifies factors necessary for the regenerative phenotype of prostate luminal epithelial progenitors.
Moline DC, Zenner ML, Burr A, Vellky JE, Nonn L, Vander Griend DJ.
Am J Clin Exp Urol. 2022 Dec 25;10(6):425-439. eCollection 2022. PMID: 36636696 - Advanced glycation end-products (AGEs) are lower in prostate tumor tissue and inversely related to proportion of West African ancestry.
Zenner ML, Helou YB, Deaton RJ, Sverdlov M, Wang H, Kajdacsy-Balla A, Macias V, Voisine C, Murray M, Abdulkadir SA, Murphy AB, Nonn L. Prostate. 82(3):306-313, 2021.
PMID: 34855273 - Keratin Profiling by Single-Cell RNA-Sequencing Identifies Human Prostate Stem Cell Lineage Hierarchy and Cancer Stem-Like Cells.
Hu WY, Hu DP, Xie L, Nonn L, Lu R, Abern M, Shioda T, Prins GS. Int J Mol Sci. 2021 Jul 28;22(15):8109. doi: 10.3390/ijms22158109.
PMID: 34360875 - Vitamin D sufficiency enhances differentiation of patient-derived prostate epithelial organoids.
McCray T, Pacheco JV, Loitz CC, Garcia J, Baumann B, Schlicht MJ, Valyi-Nagy K, Abern MR, Nonn L. iScience. 24(1):101974 2021
PMID: 33458620 - Oncogenic and tumor-suppressive microRNAs in prostate cancer.
Zenner ML, Baumann B, Nonn L. Curr Opin Endocr Metab Res. 10:50-59, 2020
PMID: 3304316 - Vitamin D regulates prostate cell metabolism via genomic and non-genomic mitochondrial redox-dependent mechanisms.
Blajszczak CC, Nonn L. J Steroid Biochem Mol Biol.. Epub 2019 Sep 28.
PMID: 31574299 - Single-cell RNA-Seq analysis identifies a putative epithelial stem cell population in human primary prostate cells in monolayer and organoid culture conditions.
McCray T, Moline D, Baumann B, Vander Griend DJ, Nonn L. Am J Clin Exp Urol. 7(3):123-138, 2019
PMID: 31317052 - High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.
Baumann B, Lugli G, Gao S, Zenner M, Nonn L. Prostate, 79(8):840-855, 2019.
PMID: 30905091 - Genetic ancestry analysis reveals misclassification of commonly used cancer cell lines.
Hooker SE, Woods-Burnham L, Bathina M, Lloyd SM, Gorjala P, Mitra R, Nonn L, Kimbro KS, Kittles R. Cancer Epidemiol Biomarkers Prev. [Epub ahead of print], 2019
PMID: 30787054 - Prostate Stroma Increases the Viability and Maintains the Branching Phenotype of Human Prostate Organoids.
Richards Z*, McCray T*, Marsili J, Zenner ML, Manlucu JT, Garcia J, Murray M., Voisine CM, Murphy AB, Abdulkadir SA, Prins GS, Nonn L. iScience, 12:304-317, 2019
PMID: 30735898 - Association of High miR-182 Levels with Low-Risk Prostate Cancer.
Baumann B, Acosta AM, Richards Z, Deaton R, Kajdacsy-Balla A, Gann P, Nonn L. Am J Path,[Epub ahead of print]
PMID: 30703341 - Handling and Assessment of Human Primary Prostate Organoid Culture.
McCray TM, Richards Z, Marsili J, Prins GS, Nonn L. J Vis Exp, Jan 17;(143), 2019
PMID: 30735176 - GPX1 Localizes to the Nucleus in Prostate Epithelium and its Levels are not Associated with Prostate Cancer Recurrence.
Ekoue DN, Ansong E, Hong LK, Nonn L, Macias V, Deaton R, Rupnow R, Gann PH, Kajdacsy-Balla A, Diamond AM. Antioxidants, Nov 18;7(11), 2018.
PMID: 30453672 - Cell-type specific expression of oncogenic and tumor suppressive microRNAs in the human prostate and prostate cancer.
Kumar B, Rosenberg AZ, Choi SM, Fox-Talbot K, De Marzo AM, Nonn L, Brennen WN, Marchionni L, Halushka MK, Lupold SE. Sci Rep, May 8;8(1):7189, 2018.
PMID: 29739972 - Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer.
Ekoue DN, Ansong E, Liu L, Macias V, Deaton R, Lacher C, Picklo M, Nonn L, Gann PH, Kajdacsy-Balla A, Prins GS, Freeman VL, Diamond AM. Prostate, Mar;78(4):279-288, 2018
PMID: 29314169 - Organoids model distinct Vitamin E effects at different stages of prostate cancer evolution.
Njoroge RN, Unno K, Zhao JC, Naseem AF, Anker JF, McGee WA, Nonn L, Abdulkadir SA. Sci Rep, Nov 24;7(1):16285, 2017
PMID: 29176677 - The miR-183 family cluster contains a secondary transcription site and induces oncomiR-like changes in zinc and prostate growth.
Damba S, Baumann B, McCray T, Williams L, Richards Z, Prins G, Nonn L. Sci Rep, 7(1):7704, 2017.
PMCID: PMC5550464 - Isolation and functional interrogation of adult human prostate epithelial stem cells at single cell resolution.
Hu WY, Hu DP, Xie L, Li Y, Majumdar S, Nonn L, Hu H, Shioda T, Prins GS. Stem Cell Res. 23:1-12, 2017
PMID: 28651114 - Modeling African American prostate adenocarcinoma by inducing defined genetic alterations in organoids.
Unno K, Roh M, Yoo YA, Al-Shraideh Y, Wang L, Nonn L, Abdulkadir SA. Oncotarget. 2017 Apr 19. Epub ahead of print
PMID: 28472770 - Prostatic Compensation of the Vitamin D Axis in African American men.
Richards Z, Batai K, Farhat R, Makowski A, Gann PH, Kittles R, Nonn L. JCI Insight, 2(2):e91054, 2017.
PMID: 28138564 - microRNAs and DICER1 are regulated by 1,25-dihydroxyvitamin D in prostate stroma.
Dambal S, Giangreco AA, Acosta AM, Fairchild A, Richards Z, Deaton R, Wagner D, Vieth R, Gann PH, Kajdacsy-Balla A, Van der Kwast T, Nonn L. J Steroid Biochem Mol Biol. 2017 [Epub ahead of print]
PMID: 28089917 - miR-182, of the miR-183 cluster family, is packaged in exosomes and is detected in human exosomes from serum, breast cells and prostate cells.
Mihelich BL, Dambal S, Lin S, Nonn L. Oncol Lett. 2016 Aug;12(2):1197-1203.
PMID: 27446418 - Vitamin D and Immune Response: Implications for Prostate Cancer in African Americans.
Batai K, Murphy AB, Nonn L, Kittles RA. Front Immunol. 2016 Feb 22;7:53.
PMID: 26941739 - Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis.
Lugli G, Kataria Y, Richards Z, Ma J, Gann P, Zhou XF and Nonn L.
J Vis Exp. 2015 Nov 26;(105). - A Phase II Randomized Trial of Lycopene-Rich Tomato Extract Among Men with High-Grade Prostatic Intraepithelial Neoplasia.
Gann PH, Deaton RJ, Rueter EE, van Breemen RB, Nonn L, Macias V, Han M, Ananthanarayanan
V. Nutr Cancer. 2015;67(7):1104-12. - The microRNA-183 cluster: the family that plays together stays together.
Dambal S, Shah M, Mihelich BL, Nonn L.
Nucleic Acids Res. 2015 Sep 3;43(15):7173-88. - Elevated Serum MicroRNA Levels Associate with Absence of High-Grade Prostate Cancer in a Retrospective Cohort.
Mihelich BL, Maranville JC, Nolley R, Peehl DM, Nonn L. PLoS One. 2015 Apr 14;10(4):e0124245.
doi: 10.1371/journal.pone.0124245. eCollection 2015 - Differential expression and regulation of vitamin D hydroxylases and inflammatory genes in prostate stroma and epithelium by 1,25-dihydroxyvitamin D in men with prostate cancer and an in vitro model.
Giangreco AA, Dambal S, Wagner D, Van der Kwast T, Vieth R, Prins GS, Nonn L.
J Steroid Biochem Mol Biol. 2014 Oct 8. pii: S0960-0760(14)00237-4. - Inhibition of CHOP accentuates the apoptotic effect of α-mangostin from the mangosteen fruit (Garcinia mangostana) in 22Rv1 prostate cancer cells.
Li G, Petiwala SM, Nonn L, Johnson JJ.
Biochem Biophys Res Commun. 2014 Sep 26. pii: S0006-291X(14)01670-2. - Rosemary (Rosmarinus officinalis) extract modulates CHOP/GADD153 to promote androgen receptor degradation and decreases xenograft tumor growth.
Petiwala SM, Berhe S, Li G, Puthenveetil AG, Rahman O, Nonn L, Johnson JJ.
PLoS One. 2014 Mar 5;9(3):e89772. doi: 10.1371/journal.pone.0089772. eCollection 2014 - Selective modulation of endoplasmic reticulum stress markers in prostate cancer cells by a standardized mangosteen fruit extract.
Li G, Petiwala SM, Pierce DR, Nonn L, Johnson JJ.
PLoS One. 2013 Dec 18;8(12):e81572. doi: 10.1371/journal.pone.0081572. eCollection 2013. - Tumor suppressor microRNAs, miR-100 and -125b, are regulated by 1,25-dihydroxyvitamin D in primary prostate cells and in patient tissue.
Giangreco AA, Vaishnav A, Wagner D, Finelli A, Fleshner N, Van der Kwast T, Vieth R, Nonn L.
Cancer Prev Res (Phila). 2013 May;6(5):483-94. - Effects of lycopene on protein expression in human primary prostatic epithelial cells.
Qiu X, Yuan Y, Vaishnav A, Tessel MA, Nonn L, van Breemen
RB. Cancer Prev Res (Phila). 2013 May;6(5):419-27. - Randomized clinical trial of vitamin D3 doses on prostatic vitamin D metabolite levels and ki67 labeling in prostate cancer patients.
Wagner D, Trudel D, Van der Kwast T, Nonn L, Giangreco AA, Li D, Dias A, Cardoza M, Laszlo S, Hersey K, Klotz L, Finelli A, Fleshner N, Vieth R.
J Clin Endocrinol Metab. 2013 Apr;98(4):1498-507. - The sum of many small changes: microRNAs are specifically and potentially globally altered by vitamin D3 metabolites.
Giangreco AA, Nonn L.
J Steroid Biochem Mol Biol. 2013. 136:86-93. Review. - miR-183-96-182 cluster is overexpressed in prostate tissue and regulates zinc homeostasis in prostate cells.
Mihelich BL, Khramtsova EA, Arva N, Vaishnav A, Johnson DN, Giangreco AA, Martens-Uzunova E, Bagasra O, Kajdacsy-Balla A, Nonn L.
J Biol Chem. 2011. 286(52):44503-11. - Gene expression patterns in the human breast after pregnancy.
Asztalos S, Gann PH, Hayes MK, Nonn L, Beam CA, Dai Y, Wiley EL, Tonetti DA.
Cancer Prev Res (Phila). 2010. 3(3):301-11. - mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials.
Nonn L, Vaishnav A, Gallagher L, Gann PH.
Exp Mol Pathol. 2010. 88(1):45-51. - Evidence for field cancerization of the prostate.
Nonn L, Ananthanarayanan V, Gann PH.
Prostate. 2009. 69(13):1470-9. Review. - Calcitriol as a chemopreventive and therapeutic agent in prostate cancer: role of anti-inflammatory activity.
Krishnan AV, Moreno J, Nonn L, Swami S, Peehl DM, Feldman D.
J Bone Miner Res. 2007. 22 Suppl 2:V74-80.Review. - Novel pathways that contribute to the anti-proliferative and chemopreventive activities of calcitriol in prostate cancer.
Krishnan AV, Moreno J, Nonn L, Malloy P, Swami S, Peng L, Peehl DM, Feldman D.
J Steroid Biochem Mol Biol. 2007 Mar;103(3-5):694-702. - Chemopreventive anti-inflammatory activities of curcumin and other phytochemicals mediated by MAP kinase phosphatase-5 in prostate cells.
Nonn L, Duong D, Peehl DM.
Carcinogenesis. 2007 Jun;28(6):1188-96. - Inhibition of p38 by vitamin D reduces interleukin-6 production in normal prostate cells via mitogen-activated protein kinase phosphatase 5: implications for prostate cancer prevention by vitamin D.
Nonn L, Peng L, Feldman D, Peehl DM.
Cancer Res. 2006 Apr 15;66(8):4516-24. - Tumor-selective killing by selenite in patient-matched pairs of normal and malignant prostate cells.
Husbeck B, Nonn L, Peehl DM, Knox SJ.
Prostate. 2006 Feb 1;66(2):218-25. - Regulation of prostaglandin metabolism by calcitriol attenuates growth stimulation in prostate cancer cells.
Moreno J, Krishnan AV, Swami S, Nonn L, Peehl DM, Feldman D.
Cancer Res. 2005 Sep 1;65(17):7917-25. - Molecular targets of doxazosin in human prostatic stromal cells.
Zhao H, Lai F, Nonn L, Brooks JD, Peehl DM.
Prostate. 2005 Mar 1;62(4):400-10. - Molecular activity of 1,25-dihydroxyvitamin D3 in primary cultures of human prostatic epithelial cells revealed by cDNA microarray analysis.
Peehl DM, Shinghal R, Nonn L, Seto E, Krishnan AV, Brooks JD, Feldman D.
J Steroid Biochem Mol Biol. 2004 Oct;92(3):131-41. - Mechanisms of decreased Vitamin D 1alpha-hydroxylase activity in prostate cancer cells.
Ma JF, Nonn L, Campbell MJ, Hewison M, Feldman D, Peehl DM.
Mol Cell Endocrinol. 2004 Jun 30;221(1-2):67-74. - Increased expression of mitochondrial peroxiredoxin-3 (thioredoxin peroxidase-2) protects cancer cells against hypoxia and drug-induced hydrogen peroxide-dependent apoptosis.
Nonn L, Berggren M, Powis G.
Mol Cancer Res. 2003 Jul;1(9):682-9. - The absence of mitochondrial thioredoxin 2 causes massive apoptosis, exencephaly, and early embryonic lethality in homozygous mice.
Nonn L, Williams RR, Erickson RP, Powis G.
Mol Cell Biol. 2003 Feb;23(3):916-22.