Our laboratory is located on the 5th floor of Clinical Science Building within the Department of Surgery, Division of Cardiothoracic Surgery at the University of Illinois at Chicago. The laboratory is comprised of approximately 800 square feet with a dedicated tissue culture room. The tissue culture lab is certified for BL2+ for lentiviral work. The tissue culture room is equipped with two 4 feet tissue culture laminar flow hoods, 4 autoclavable tissue culture incubators, an EVOS XL core inverted microscope, an EVOS M5000 inverted fluorescent microscope, a tissue culture centrifuge, 4C° refrigerators, an AMAXA Nucleofector, 2 Countess cell counters and networked iPads.
The main lab space is equipped with 4 working benches for research specialists, postdoctoral fellows and students. The laboratory is equipped with Apple Dell and HP computers with sufficient computing power and data analysis software to process, analyze and plot the data. These computers are connected to the University of Illinois network and therefore facilitate our access to the University of Illinois System Library Collection and other data banks as required. One of the desktop computers is installed with Snap Gene software to record sequences and vector maps. Standard laboratory equipment includes 4C° refrigerators, -20C° freezers, refrigerated microcentrifuges, a gradient PCR thermocycler, nano-drop, a fluorometer, Gel-Doc EZ gel documentation system, iBright Western blot imaging system, turbo blot system, Luminex FLEXMAP 3D system, Luminex Guava EasyCyte benchtop cytometer, QuantStudio real-time PCR system and all equipment necessary for molecular biology and functional assays.
Our laboratory has two -80C° freezers and two cryopreservation tanks in the shared core space. The research division of the Department of Surgery provides core space for an Agilent Seahorse Analyzers with complete supporting equipment, qRT-PCR, incubators for bacterial cultures, a high-speed centrifuge, spectrophotometer, multi-functional plate reader, phosphoimager, stereotaxic instrument, sonicator, pH electrodes and a Milli‐Q water purification system. A fully motorized fluorescence microscope from Nikon capable of taking live cell imaging with analysis software is also available in the department.